MacConkey Broth EP/USP, Syrup + Durham Tube

A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli
from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin
peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a
selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A
color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such
as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective
enrichment broth, with subculture performed onto MacConkey Agar.

Specifications

Container Syrup
Available fill size(s) (ml) 50, 100
Neck width (mm) 24
Caps PP, Perf, Metal
Shelf life 12 months
Storage Temp 2-25oC
Appearance Purple liquid with Durham's tube
Recommended Incubation 37/44oC
pH 7.35 +/-0.15

Quality Control

Organisms Ref. No Result
Escherichia coli ATCC 8739 NCIB 8545 Turbidity, acid production and gas formation from an inoculum of <100cfu
Staphylococcus aureus ATCC 6538 NCTC 10788 No growth from an inoculum of >1000cfu

Supporting Documents

MSDS Download
Technical data sheet Download

Description

0523

SKU: 0523